Description

ATP is the primary energy currency of living systems. Virtually all energy dependent processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in mitochondria and a variety of genetic diseases affect ATP formation in the mitochondria. There are many commercially available ATP assays which detect femtomoles or less of ATP using luminescence, but these kits require specialized instrumentation and utilize luciferase which can be difficult to maintain in active form. The ATP Assay kit is designed to be a robust, simple method that determines ATP concentration by phosphorylating glycerol (Glycero-6 phosphate) resulting in a colorimetric (OD = 570 nm) or fluorometric (Ex/Em = 535/590 nm) product proportional to the amount of ATP present.

 

Kit contents

Calculation

Concentration of ATP (C) = B/V x D (nmol/ ㎕ or mM )

Citation

1) Kim, S., Oh, M., Kang, M., and Ko, J. (2022). Small leucine zipper protein functions as a modulator for metabolic reprogramming of colorectal cancer cells by inducing nutrient stress-mediated autophagy. Cellular and Molecular Life Sciences 79, 505. 10.1007/s00018-022-04535-4.

2) Song, N., Park, M., Kim, N., Lee, Y., Jung, E., and Lee, D. (2022). Tumor-targeting oxidative stress nanoamplifiers as anticancer nanomedicine with immunostimulating activity. Biomaterials Science 10, 6160-6171. 10.1039/D2BM00601D.

3) Sukhan, Z.P., Hossen, S., Cho, Y., Lee, W.K., and Kho, K.H. (2022). Hdh-Tektin-4 Regulates Motility of Fresh and Cryopreserved Sperm in Pacific Abalone, Haliotis discus hannai. Front Cell Dev Biol 10, 870743. 10.3389/fcell.2022.870743.

4) Lim, S.G., Suk, K., and Lee, W.H. (2020). LETMD1 Regulates Phagocytosis and Inflammatory Responses to Lipopolysaccharide via Reactive Oxygen Species Generation and NF-kappaB Activation in Macrophages. J Immunol 204, 1299-1309. 10.4049/jimmunol.1900551.

5) Chang, Y.B., Hong, K.B., Kim, M.G., Suh, H.J., and Jo, K. (2021). Effect of the protein hydrolysate of rice syrup meal on the endurance exercise performance of BALB/c mice. Food Funct 12, 1338-1348. 10.1039/d0fo02667k.

6) Sharma, S., Lee, K.-J., Bajgai, J., Trinh, T.T., Antonio, J.M., Rahman, M.H., Vira, K., Sofian, A.-N., Cho, S.H., Kim, C.-S., and Kim, Y. (2022). Anti-Oxidative and Anti-Diabetic Effects of Electrolyzed Weakly Alkaline Reduced Water on Renal Proximal Tubular Epithelial Cells. Processes 10, 2025.

7) Lee, Y., Saifuddin, M., Park, S.Y., and Kim, C.G. (2023). Synergistic effect of total residual oxidants and microplastics on seawater disinfection: An ecotoxicity study. Environmental Engineering Research 28, 210612-210610. 10.4491/eer.2021.612.10, 2025.